Species-specific sperm adhesion in sea urchins. A quantitative investigation of bindin- mediated egg agglutination
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چکیده
منابع مشابه
Species-specific sperm adhesion in sea urchins. A quantitative investigation of bindin- mediated egg agglutination
Bindin, a protein component of the acrosomal vesicle of sea urchin sperm, has been isolated from Arbacia punctulata and strongylocentrous purpuratus. Using this isolated bindin, we have devised a quantitative assay for bindin-mediated egg agglutination and compared the agglutination of bindin eggs from A. puntulata and S. purpuratus. Bindin- mediated agglutination is species -specific in both s...
متن کاملSpecies-specific Sperm Adhesion in Sea Urchins
Bindin, a protein component of the acrosomal vesicle of sea urchin sperm, has been isolated from Arbacia punctulata and Strongylocentrotus purpuratus . Using this isolated bindin, we have devised a quantitative assay for bindin-mediated egg agglutination and compared the agglutination of bindin and eggs from A . punctulata and S. purpuratus . Bindin-mediated agglutination is species-specific in...
متن کاملAdaptive evolution of sperm bindin tracks egg incompatibility in neotropical sea urchins of the genus Echinometra.
Bindin is a gamete recognition protein known to control species-specific sperm-egg adhesion and membrane fusion in sea urchins. Previous analyses have shown that diversifying selection on bindin amino acid sequence is found when gametically incompatible species are compared, but not when species are compatible. The present study analyzes bindin polymorphism and divergence in the three closely r...
متن کاملCarbohydrate specificity of sea urchin sperm bindin: a cell surface lectin mediating sperm-egg adhesion
We have examined the carbohydrate specificity of bindin, a sperm protein responsible for the adhesion of sea urchin sperm to eggs, by investigating the interaction of a number of polysaccharides and glycoconjugates with isolated bindin. Several of these polysaccharides inhibit the agglutination of eggs by bindin particles. An egg surface polysaccharide was found to be the most potent inhibitor ...
متن کاملCarbohydrate Specificity of Sea a Cell Surface Lectin Mediating Urchin Sperm Bindin: Sperm-Egg Adhesion
We have examined the carbohydrate specificity of bindin, a sperm protein responsible for the adhesion of sea urchin sperm to eggs, by investigating the interaction of a number of polysaccharides and glycoconjugates with isolated bindin. Several of these polysaccharides inhibit the agglutination of eggs by bindin particles. An egg surface polysaccharide was found to be the most potent inhibitor ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1979
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.83.3.595